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The yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. It is ideally suited for large-scale production of recombinant eukaryotic proteins.Our yeast expression system provides the advantages of both prokaryotic and eukaryotic expression systems: high expression level, low cost, ease of scale up, and proper protein folding. We have multiple strains and expression vectors to help identify the highest yielding conditions. We also utilize robust secretion signal sequences to ensure the highest secretion of recombinant protein. Our customers can also choose constitutive or inducible expression systems.
We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a yeast expression vector. Once cloned, all constructs are restriction digest and sequence verified.
The appropriate yeast strain is transformed with the linearized expression construct and positively selected transformants screened for integration by PCR. Small scale induction experiments are conducted to identify high-expressing transformants and optimize expression and purification conditions.
We use our optimized conditions to scale up expression to one liter cultures. We access multiple purification strategies to isolate several milligrams of product at this stage. If larger quantities are required, we can scale up to 35-500L culture sizes.