Baculovirus Expression System | Protein Services

The baculovirus expression vector system (BEVS) is a powerful system for the production of high quality proteins. Although time lines are increased compared to E. coli, proteins produced in BEVS tend to be soluble and functional and carry post-translational modifications like phosphorylation and glycosylation. The system gives very high recombinant protein yields and we often recommend it for proteins that are difficult to express in E. coli.

Phase I: Gene Synthesis, Subcloning, and Baculovirus Preparation

We can take a customer provided cDNA or optimize and synthesize any desired coding sequence and subclone it into a baculovirus transfer vector. Once cloned, all constructs are restriction digest and sequence verified. Transfer vectors are used to generate recombinant bacmids and P1, P2, and P3 virus stocks.

Phase II: Protein Expression and Purification

We infect insect cells with high titer P3 viral stock and determine optimal conditions for protein expression and purification.

Phase III: Larger Scale Protein Expression and Purification

Should additional quantities of protein be required we scale up expression and purification based on optimized conditions.