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Our customer service representatives are available 24 hours, Monday through Friday to assist you.| Introduction | The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes throµgh its enzymatic conjµgation to other cellular proteins.Ubiquitination occurs throµgh sequential steps catalyzed by activating (E1), conjµgating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub’s C-terminal glycine residue (G76) and a lysine residue of the target protein, althoµgh N-terminal ubiquitination is also known.Outcomes of this modification include destabilization of the conjµgated protein, altered protein trafficking and functional modulation.After targeting the protein for specific localizations, ubiquitin is released from the substrate by deubiquitinating enzymes.A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process.As consequence, the Ub derivative becomes irreversibly conjµgated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjµgation, at the expense of the free form. |
| Synonyms | Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin G76A. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | Diluted in PBS plus 5% glycerol. |
| Stability | Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Usage | NeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals. |
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