TRXR E.Coli-Reductase

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TRXR E.Coli

Qty


Total
$65
Catalog #
ENPS-514
Size
Description
TRXR E.coli Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 321 amino acids(1-321 a.a.) and having a molecular mass of 34.6 kDa. TRXR protein is purified by standard chromatography.
Additional Information
IntroductionTRXR is a ubiquitous enzyme which participates in various cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase cleaves thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxidec.
SynonymsTRXB, TRXR, Thioredoxin Reductase.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationTRXR E.Coli solution containing 20mM Tris HCl pH-8, 1mM DTT, and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGTTKHSKLL
ILGSGPAGYT
AAVYAARANL
QPVLITGMEK
GGQLTTTTEV
ENWPGDPNDL
TGPLLMERMH
EHATKFETEI
IFDHINKVDL
QNRPFRLNGD
NGEYTCDALI
IATGASARYL
GLPSEEAFKG
RGVSACATCD
GFFYRNQKVA
VIGGGNTAVE
EALYLSNIAS
EVHLIHRRDG
FRAEKILIKR
LMDKVENGNI
ILHTNRTLEE
VTGDQMGVTG
VRLRDTQNSD
NI
PurityGreater than 90% as determined by SDS-PAGE.
Biological ActivitySpecific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
UsageNeoBiolab's products are furnished forLABORATORY RESEARCHUSEONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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