TARS Human-Synthetase

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TARS Human

Qty


Total
$65
Catalog #
ENPS-578
Size
Description
TARS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 743 amino acids (1-723) and having a molecular mass of 85.6kDa.TARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionThreonyl-tRNA synthetase, cytoplasmic (TARS) is a member of the class-II aminoacyl-tRNA synthetase family. The main role of TARS is in tRNA aminoacylation. The N-terminal domain of the TARS enzyme is responsible for the competition with the ribosome whereas the catalytic and the C-terminal domain are involved in binding the 2 anticodon arm-like structures in the operator.
SynonymsThreonine--tRNA ligase, cytoplasmic, Threonyl-tRNA synthetase, ThrRS, TARS.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe TARS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol and 150mM NaCl.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MFEEKASSPS
GKMGGEEKPI
GAGEEKQKEG
GKKKNKEGSG
DGGRAELNPW
PEYIYTRLEM
YNILKAEHDS
ILAEKAEKDS
KPIKVTLPDG
KQVDAESWKT
TPYQIACGIS
QGLADNTVIA
KVNNVVWDLD
RPLEEDCTLE
LLKFEDEEAQ
AVYWHSSAHI
MGEAMERVYG
GCLCYGPPIE
NGFYYDMYLE
EGGVSSNDFS
SLEALCKKII
KE
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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