SURA E.Coli-Isomerase

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SURA E.Coli

Qty


Total
$65
Catalog #
ENPS-264
Size
Description
SURA E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 429 amino acids (21-428 a.a.) and having a molecular weight of 47.3kDa. The SURA is fused to 20 a.a His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Additional Information
IntroductionSURA is a PPIase enzyme and chaperone of Escherichia coli and other Gram-negative bacteria. SURA is a key player in the biogenesis of beta-barrel outer membrane proteins and is involved in cell envelope homeostasis and cell envelope functions. SURA is necessary for the survival of E.coli in stationary phase and needed for pilus biogenesis.
SynonymsRotamase surA, Survival protein A.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe SURA 1mg/ml protein solution contains 20mM Tris-HCl, pH-8, and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MAPQVVDKVA
AVVNNGVVLE
SDVDGLMQSV
KLNAAQARQQ
LPDDATLRHQ
IMERLIMDQI
ILQMGQKMGV
KISDEQLDQA
IANIAKQNNM
TLDQMRSRLA
YDGLNYNTYR
NQIRKEMIIS
EVRNNEVRRR
ITILPQEVES
LAQQVGNQND
ASTELNLSHI
LIPLPENPTS
DQVNEAESQA
RAIVDQARNG
ADFGKLAIAH
SADQQALNGG
QM
PurityGreater than 95.0% as determined by SDS-PAGE.
Biological ActivitySpecific activity is > 450 nmoles/min/µg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
UsageNeoBiolab''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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