SlyD E.Coli-Isomerase

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SlyD E.Coli

Qty


Total
$65
Catalog #
ENPS-345
Size
Description
SlyD Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids and having a molecular mass of 21 kDa.
Additional Information
IntroductionSlyD accessiton#: NP_755987 is a putative folding helper protein from the Escherichia coli cytosol, which has N-terminal prolyl isomerase domain of the FKBP type and a most likely unstructured C-terminal tail. SlyD is an important factor in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
SynonymsFKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase, SlyD.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationSlyD protein solution contains 20mM Tris pH-7.5.
StabilitySlyD althoµgh stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMKVAKDLVVS
LAYQVRTEDG
VLVDESPVSA
PLDYLHGHGS
LISGLETALE
GHEVGDKFDV
AVGANDAYGQ
YDENLVQRVP
KDVFMGVDEL
QVGMRFLAET
DQGPVPVEIT
AVEDDHVVVD
GNHMLAGQNL
KFNVEVVAIR
EATEEELAHG
HVHGAHDHHH
DHDHDGCCGG
HGHDHGHEHG
GEGCCGGKGN
GGCGCH.
PurityGreater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivitySpecific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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