PSMB5 Human-Proteasome

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PSMB5 Human

Qty


Total
$65
Catalog #
ENPS-057
Size
Description
PSMB5 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 241 amino acids (60-263 a.a.) and having a molecular mass of 26.7kDa. The PSMB5 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionPSMB5 belongs to the proteasome B-type family that has a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is substituted by catalytic subunit 3i (proteasome beta 8 subunit). A fundamental function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB5 exhibits an ATP dependent proteolytic activity and is involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.
SynonymsProteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X, MGC104214.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe PSMB5 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMRGSHHHHHH
GMASMTGGQQ
MGRDLYDDDD
KDRWGSMTTT
LAFKFRHGVI
VAADSRATAG
AYIASQTVKK
VIEINPYLLG
TMAGGAADCS
FWERLLARQC
RIYELRNKER
ISVAAASKLL
ANMVYQYKGM
GLSMGTMICG
WDKRGPGLYY
VDSEGNRISG
ATFSVGSGSV
YAYGVMDRGY
SYDLEVEQAY
DLARRAIYQA
TYRDAYSGGA
VNLYHVREDG
WIRVSSDNVA
DL
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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