POLL Human-Polymerase

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POLL Human

Qty


Total
$65
Catalog #
ENPS-189
Size
Description
POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionDNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5’-deoxyribose-5-phosphate lyase activity which is synchronized by PCNA and replication protein A.
SynonymsPolymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MLMHHQKYLQ
RFLGGKREKK
QKEACSIPGI
GKRMAEKIIE
ILESGHLRKL
DHISESVPVL
ELFSNIWGAG
TKTAQMWYQQ
GFRSLEDIRS
QASLTTQQAI
GLKHYSDFLE
RMPREEATEI
EQTVQKAAQA
FNSGLLCVAC
GSYRRGKATC
GDVDVLITHP
DGRSHRGIFS
RLLDSLRQEG
FLTDDLVSQE
ENGQQQKYLG
VCRLPGPGRR
HR
PurityGreater than 90% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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