PHOSPHO2 Human-Phosphatase

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PHOSPHO2 Human

Qty


Total
$65
Catalog #
ENPS-238
Size
Description
PHOSPHO2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-241) and having a molecular mass of 30.3kDa.PHOSPHO2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionPyridoxal phosphate phosphatase PHOSPHO2, orphan 2 (PHOSPHO2) is a member of the haloacid dehalogenase (HAD) superfamily. Phosphatase has an elevated activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralizing cells and is believed to be implicated in the production of inorganic phosphate for bone mineralization. PHOSPHO2 is a recognized phosphatase sharing a 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are especially similar, however surprisingly recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine comparatively inadequately.
SynonymsPyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe PHOSPHO2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMKILLV
FDFDNTIIDD
NSDTWIVQCA
PNKKLPIELR
DSYRKGFWTE
FMGRVFKYLG
DKGVREHEMK
RAVTSLPFTP
GMVELFNFIR
KNKDKFDCII
ISDSNSVFID
WVLEAASFHD
IFDKVFTNPA
AFNSNGHLTV
ENYHTHSCNR
CPKNLCKKVV
LIEFVDKQLQ
QGVNYTQIVY
IGDGGNDVCP
VTFLKNDDVA
MPRKGYTLQK
TL
PurityGreater than 85.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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