PGPEP1 Human-Peptidase

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PGPEP1 Human

Qty


Total
$65
Catalog #
ENPS-679
Size
Description
PGPEP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-209) and having a molecular mass of 25.5kDa.PGPEP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionPyroglutamyl-Peptidase I (PGPEP1) is an omega peptidase which detaches pyroglutamyl residues from the amino termini of peptides and proteins. PGPEP1 is a cytosolic cysteine peptidase which is expressed in most cell types. PGPEP1 enzyme has need of s a thiol-reducing agent for activity. PGPEP1 is possibly involved in the inactivation of biologically active peptides which have an amino terminal pyroglutamyl group, for instance peptides as neurotensin, luteinizing hormone releasing hormone, and thyrotropinreleasing hormone.
SynonymsPyroglutamyl-peptidase 1, EC 3.4.19.3, 5-oxoprolyl-peptidase, Pyroglutamyl aminopeptidase I, PAP-I, Pyroglutamyl-peptidase I, PGP-I, Pyrrolidone-carboxylate peptidase, PGPEP1, PGPI, PGP, Pcp.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe PGPEP1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMEQPRKA
VVVTGFGPFG
EHTVNASWIA
VQELEKLGLG
DSVDLHVYEI
PVEYQTVQRL
IPALWEKHSP
QLVVHVGVSG
MATTVTLEKC
GHNKGYKGLD
NCRFCPGSQC
CVEDGPESID
SIIDMDAVCK
RVTTLGLDVS
VTISQDAGRY
LCDFTYYTSL
YQSHGRSAFV
HVPPLGKPYN
ADQLGRALRA
IIEEMLDLLE
QSEGKINYCH
KH
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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