For quotations, please use our online quotation form, and you may also contact us by
service@kendallscientific.com
+1-888.733.6849 (Toll-free)
+1-617.299.7367 (Int’l))
+1-888.733.6849
Our customer service representatives are available 24 hours, Monday through Friday to assist you.Introduction | Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy. |
Synonyms | CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered white lyophilized powder. |
Formulation | The CXCL4 protein was lyophilized after extensive dialysis against 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer. |
Solubility | It is recommended to reconstitute the lyophilized CXCL4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized CXCL4 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Amino Acid Sequence | The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Biological Activity | The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 |
Usage | NeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs,agricultural or pesticidal products, food additives or household chemicals. |
N/A
N/A