MCEE Human-Epimerase

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MCEE Human

Qty


Total
$65
Catalog #
ENPS-020
Size
Description
MCEE produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids (37-176a.a.) and having a molecular mass of 17.3kDa.MCEE is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionMCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA throµghout the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. MCEE protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria can appear in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.
SynonymsGLOD2, Methylmalonyl CoA Epimerase, Glyoxalase Domain Containing 2, DL-methylmalonyl-CoA Racemase.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered clear solution.
FormulationThe MCEE protein solution (1mg/1ml) is formulated 20 mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 1mM DTT, 0.1mM PMSF and 10% glycerol.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MQVTGSVWNL
GRLNHVAIAV
PDLEKAAAFY
KNILGAQVSE
AVPLPEHGVS
VVFVNLGNTK
MELLHPLGRD
SPIAGFLQKN
KAGGMHHICI
EVDNINAAVM
DLKKKKIRSL
SEEVKIGAHG
KPVIFLHPKD
CGGVLVELEQ
A
PurityGreater than 90% as determined by SDS-PAGE.
UsageNeoBiolabs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
StorageStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Background

N/A

Protocol

N/A

MSDS
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