KLK11 Human-Kallikrein

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KLK11 Human

Qty


Total
$65
Catalog #
ENPS-518
Size
Description
KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of25.6kDa thoµgh on SDS-PAGE migrates at about 41kDa due to the glycosilation.The KLK11 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionKLK11 is a multifunctional protease. KLK11 cleaves ''bz-Phe-Arg-4-methylcoumaryl-7-amide'', KLK11 is a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine rather than lysine residues.
Synonymskallikrein-related peptidase 11, TLSP, hippostasin, Kallikrein-11, hK11, Hippostasin, Trypsin-like protease, Serine protease 20, PRSS20, MGC33060, EC 3.4.21.
SourceHEK293-F Cells.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe KLK11 protein was lyophilized from a 0.2
SolubilityIt is recommended to reconstitute the lyophilized KLK11 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized KLK11 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK11 should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceETRIIKGFEC
KPHSQPWQAA
LFEKTRLLCG
ATLIAPRWLL
TAAHCLKPRY
IVHLGQHNLQ
KEEGCEQTRT
ATESFPHPGF
NNSLPNKDHR
NDIMLVKMAS
PVSITWAVRP
LTLSSRCVTA
GTSCLISGWG
STSSPQLRLP
HTLRCANITI
IEHQKCENAY
PGNITDTMVC
ASVQEGGKDS
CQGDSGGPLV
CNQSLQGIIS
WGQDPCAITR
KPGVYTKVCK
YVDWIQETMK
NN
PurityGreater than 95.0% as determined by SDS-PAGE.
Biological ActivityKLK11 specific activity is greater than 2200 pmoles/min/μg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 μg of activated enzyme. The reaction is carried out in a volume of 100μL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5’Dithio-bis(2-nitrobenzoic acid)), pH8.5 at 37°C. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1.
UsageNeoBiolab's products are furnished forLABORATORY RESEARCHUSEONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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