Intein Bacillus Circulans-Other

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Intein Bacillus Circulans

Qty


Total
$65
Catalog #
PRPS-965
Size
Description
Intein Bacillus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 533 amino acids (3-518) and having a molecular mass of 59.4 kDa.Intein is fused to a 16 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionIntein is a section of a protein which can remove itself and return the remaining segment with a peptide bond. In addition, Inteins hold an endonuclease domain which takes part in Intein proliferation. Actually, various genes have unrelated intein-coding segments inserted at altered positions and they were found in all three domains of life (eukaryotes, bacteria, and archaea) and in viruses.
SynonymsIntein-CBD
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe Intein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMKIEEGKLVI
GSLEGCFAKG
TNVLMADGSI
ECIENIEVGN
KVMGKDGRPR
EVIKLPRGRE
TMYSVVQKSQ
HRAHKSDSSR
EVPELLKFTC
NATHELVVRT
PRSVRRLSRT
IKGVEYFEVI
TFEMGQKKAP
DGRIVELVKE
VSKSYPISEG
PERANELVES
YRKASNKAYF
EWTIEARDLS

LLGSHVRKAT
YQTYAPILYE
NDHFFDYMQK
SKFHLTIEGP
KVLAYLLGLW
I
PurityGreater than 95% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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