IFN b 1a Human-Interferon

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IFN b 1a Human

Qty


Total
$65
Catalog #
CYPS-243
Size
Description
Interferon-beta 1a Human Recombinant produced in CHO (Chinese Hamster Ovarian) cells is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500 Dalton.IFN-beta1a is purified by proprietary chromatographic techniques.
Additional Information
IntroductionInterferon-beta has antiviral, antibacterial and anticancer activities.
SynonymsLeukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1a ,MGC96956.
SourceCHO (Chinese Hamster Ovarian) cells.
FormulationLyophilized from a solution (1mg/3.4ml) containing 50mM Acetate acid pH 3.8.
SolubilityIt is recommended to reconstitute the lyophilized Interferon beta 1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Interferon-beta 1a althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Beta1a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Amino Acid SequenceMSYNLLGFLQ
RSSNFQCQKL
LWQLNGRLEY
CLKDRMNFDI
PEEIKQLQQF
QKEDAALTIY
EMLQNIFAIF
RQDSSSTGWN
ETIVENLLAN
VYHQINHLKT
VLEEKLEKED
FTRGKLMSSL
HLKRYYGRIL
HYLKAKEYSH
CAWTIVRVEI
LRNFYFINRL
TGYLRN.
PurityGreater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological ActivityThe specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 106 IU/mg.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
ReferencesTitle:Interferon-? induces apoptosis in human SH-SY5Y neuroblastoma cells throµgh activation of JAK–STAT signaling and down-regulation of PI3K/Akt pathway.Publication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046.xLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2010.07046.x/full
Background

N/A

Protocol

N/A

MSDS
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