IFN a 1a Human-Interferon

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IFN a 1a Human

Qty


Total
$65
Catalog #
CYPS-527
Size
Description
Interferon-alpha 1a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19.4 kDa.The Interferon-alpha 1a contains Valine residue at position 114.The IFN-a 1a is purified by proprietary chromatographic techniques.
Additional Information
IntroductionAt least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
SynonymsInterferon-alpha 1a, IFN-a 1a, IFN alpha 1a.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a (1 mg/ml) solution in containing 1X PBS pH-7.4.
SolubilityIt is recommended to reconstitute the lyophilized IFN alpha 1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Interferon alpha 1a althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 1a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceCDLPETHSLD
NRRTLMLLAQ
MSRISPSSCL
MDRHDFGFPQ
EEFDGNQFQK
APAISVLHEL
IQQIFNLFTT
KDSSAAWDED
LLDKFCTELY
QQLNDLEACA
MQEERVGETP
LMNVDSILAV
KKYFRRITLY
LTEKKYSPCA
WEVVRAEIMR
SLSLSTNLQE
RLRRKE.
PurityGreater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe specific activity as determined in a viral resistance assay viral ressistance assay was found to be 100,000,000IU/ mg.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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