GSS Human-Synthetase

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GSS Human

Qty


Total
$65
Catalog #
ENPS-028
Size
Description
GSS Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 494 amino acids (1-474 a.a.) and having a molecular mass of 54.5kDa. The GSS is purified by proprietary chromatographic techniques.
Additional Information
IntroductionGlutathione synthetase (GSS) is the second enzyme in the glutathione biosynthesis pathway. GSS catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Defects in the GSS are the cause of glutathione synthetase deficiency aka GSS deficiency or 5-oxoprolinuria or pyroglutamic aciduria, which is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
SynonymsGlutathione synthetase, GSH synthetase, GSH-S, Glutathione synthase, GSHS, MGC14098, GSS.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe GSS solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHQH
SSGLVPRGSH
MATNWGSLLQ
DKQQLEELAR
QAVDRALAEG
VLLRTSQEPT
SSEVVSYAPF
TLFPSLVPSA
LLEQAYAVQM
DFNLLVDAVS
QNAAFLEQTL
SSTIKQDDFT
ARLFDIHKQV
LKEGIAQTVF
LGLNRSDYMF
QRSADGSPAL
KQIEINTISA
SFGGLASRTP
AVHRHVLSVL
SKTKEAGKIL
SNNPSKGLAL
GIAKAWELYG
SPNALVLLIA
QE
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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