G3BP1 Human-Other

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G3BP1 Human

Qty


Total
$65
Catalog #
ENPS-055
Size
Description
G3BP1 Human Recombinant fused with an 8 amino acid His tag at C-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 474 amino acids (1-466 a.a.) and having a molecular mass of 53.2kDa.The G3BP1 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionG3BP1 belongs to the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. G3BP1 is one of the DNA-unwinding enzymes that favors partially unwound 3'-tailed substrates and is also able to unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. G3BP1 binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. In addition, G3BP1 cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR.
SynonymsRas GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP, HDH-VIII, MGC111040.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe G3BP1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMVMEKPSPLL
VGREFVRQYY
TLLNQAPDML
HRFYGKNSSY
VHGGLDSNGK
PADAVYGQKE
IHRKVMSQNF
TNCHTKIRHV
DAHATLNDGV
VVQVMGLLSN
NNQALRRFMQ
TFVLAPEGSV
ANKFYVHNDI
FRYQDEVFGG
FVTEPQEESE
EEVEEPEERQ
QTPEVVPDDS
GTFYDQAVVS
NDMEEHLEEP
VAEPEPDPEP
EPEQEPVSEI
QEEKPEPVLE
ETAPEDAQKS
SS
PurityGreater than 85.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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