BLMH Human-Hydrolase

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BLMH Human

Qty


Total
$65
Catalog #
ENPS-025
Size
Description
BLMH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 475 amino acids (1-455a.a.) and having a molecular mass of 54.7kDa.BLMH is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionBLMH is affiliate to the papain superfamily of the cysteine protease and the peptidase C1 family. BLMH is a cytoplasmic cysteinepeptidase usually found as a homohexamer. The standard physiological role of BLMH has not been determined, but it shields normal and malignant cells from the glycopeptide antitumor drµg BLM. BLMH catalyzes the inactivation of the antitumor drµg BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and in addition demonstrates general aminopeptidase activity.
SynonymsBMH, BH, BLM hydrolase, Bleomycin Hydrolase.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered clear solution.
FormulationThe BLMH protein solution (1mg/1ml) is formulated in 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MSSSGLNSEK
VAALIQKLNS
DPQFVLAQNV
GTTHDLLDIC
LKRATVQRAQ
HVFQHAVPQE
GKPITNQKSS
GRCWIFSCLN
VMRLPFMKKL
NIEEFEFSQS
YLFFWDKVER
CYFFLSAFVD
TAQRKEPEDG
RLVQFLLMNP
ANDGGQWDML
VNIVEKYGVI
PKKCFPESYT
TEATRRMNDI
LNHKMREFCI
RLRNLVHSGA
TKGEISATQD
VM
PurityGreater than 90% as determined by SDS-PAGE.
Biological ActivitySpecific activity: > 1,000 pmole/min/µg. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37C.
UsageNeoBiolabs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
StorageStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Background

N/A

Protocol

N/A

MSDS
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