BDH1 Human-Dehydrogenase

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BDH1 Human

Qty


Total
$65
Catalog #
ENPS-222
Size
Description
BDH1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (47-343) and having a molecular mass of 35.3kDa.BDH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionBDH1 belongs to the short-chain dehydrogenase/reductase gene family. BDH1 forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH1 catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
SynonymsD-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase, BDH1, SDR9C1.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe BDH1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.2M NaCl, 2mM DTT and 0.1mM PMSF.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MASAAEPVGS
KAVLVTGCDS
GFGFSLAKHL
HSKGFLVFAG
CLMKDKGHDG
VKELDSLNSD
RLRTVQLNVC
SSEEVEKVVE
IVRSSLKDPE
KGMWGLVNNA
GISTFGEVEF
TSLETYKQVA
EVNLWGTVRM
TKSFLPLIRR
AKGRVVNISS
MLGRMANPAR
SPYCITKFGV
EAFSDCLRYE
MYPLGVKVSV
VEPGNFIAAT
SLYSPESIQA
IA
PurityGreater than 85% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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