Bcl 2 Human (minus BH3 domain)-B Cell Lymphoma

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Bcl 2 Human (minus BH3 domain)

Qty


Total
$65
Catalog #
PRPS-640
Size
Description
Bcl-2 Des BH3 domain (93-107 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 and 108-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Additional Information
IntroductionBCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thoµght to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
SynonymsApoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
SolubilitySuspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
StabilityLyophilized Bcl-2 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
ApplicationsInput marker or positive control (Western Blotting).Function study.
Background

N/A

Protocol

N/A

MSDS
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