ANSA E.coli-Other

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ANSA E.coli

Qty


Total
$65
Catalog #
ENPS-126
Size
Description
ANSA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 358 amino acids (1-338 a.a.) and having a molecular mass of 39.3kDa.ANSA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionAnsA is a cytoplasmic asparaginase from E.coli involved in intracellular asparagine utilization. E.coli has 2 L-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type L asparaginase) is constitutively expressed and is obligatory for the growth of the bacteria on asparagine as the sole nitrogen source.
SynonymsL-asparaginase 1, L-asparaginase I, L-ASNase I, L-asparagine amidohydrolase I, ansA, b1767, JW1756.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe ANSA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 2mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MQKKSIYVAY
TGGTIGMQRS
EQGYIPVSGH
LQRQLALMPE
FHRPEMPDFT
IHEYTPLMDS
SDMTPEDWQH
IAEDIKAHYD
DYDGFVILHG
TDTMAYTASA
LSFMLENLGK
PVIVTGSQIP
LAELRSDGQI
NLLNALYVAA
NYPINEVTLF
FNNRLYRGNR
TTKAHADGFD
AFASPNLPPL
LEAGIHIRRL
NTPPAPHGEG
ELIVHPITPQ
PI
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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