ALKBH2 Human-Other

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ALKBH2 Human

Qty


Total
$65
Catalog #
ENPS-119
Size
Description
ALKBH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31.4kDa.ALKBH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionALKBH2 is a member of the ALKB family.ALKBH2 is expressed in the heart, colon, liver, testis, ovary, prostate and the small intestine. ALKBH2 uses iron as a cofactor and acts as a dioxygenase which catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and ?-ketoglutarate for enzymatic activity.
SynonymsAlpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2, Oxy DC1, ALKBH2, ABH2, FLJ99103, MGC90512.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationALKBH2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 30% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MDRFLVKGAQ
GGLLRKQEEQ
EPTGEEPAVL
GGDKESTRKR
PRREAPGNGG
HSAGPSWRHI
RAEGLDCSYT
VLFGKAEADE
IFQELEKEVE
YFTGALARVQ
VFGKWHSVPR
KQATYGDAGL
TYTFSGLTLS
PKPWIPVLER
IRDHVSGVTG
QTFNFVLINR
YKDGCDHIGE
HRDDERELAP
GSPIASVSFG
ACRDFVFRHK
DSRGKSPSRR
VA
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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