ACAT1 Human-Transferase

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ACAT1 Human

Qty


Total
$65
Catalog #
ENPS-672
Size
Description
ACAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 417 amino acids (34-427) and having a molecular mass of 43.8 kDa.ACAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionAcetoacetyl-CoA thiolase (ACAT1) is an enzyme member of the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. The ACAT1 enzyme catalyzes the reversible formation of acetoacetyl-CoA from 2 molecules of acetyl-CoA. ACAT1 plays a part in lipoprotein compilation and dietary cholesterol absorption. Added to its acyltransferase activity, ACAT1 acts as a ligase.
SynonymsAcetyl-CoA acetyltransferase, mitochondrial, EC 2.3.1.9, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT, THIL.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe ACAT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl, 10% glycerol and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSVSKPTLK
EVVIVSATRT
PIGSFLGSLS
LLPATKLGSI
AIQGAIEKAG
IPKEEVKEAY
MGNVLQGGEG
QAPTRQAVLG
AGLPISTPCT
TINKVCASGM
KAIMMASQSL
MCGHQDVMVA
GGMESMSNVP
YVMNRGSTPY
GGVKLEDLIV
KDGLTDVYNKIHMGSCAENT
AKKLNIARNE
QDAYAINSYT
RSKAAWEAGK
FGNEVIPVTV
TVK
PurityGreater than 95% as determined by SDS-PAGE.
UsageNeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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