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Our customer service representatives are available 24 hours, Monday through Friday to assist you.Reactivity | Human |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 350kDa |
Observed MW | Refer to Figures |
Immunogen | A phospho specific peptide corresponding to residues surrounding S1981 of human ATM |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | ep |
Synonym | AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; |
Western blot analysis of extracts of 293 cells, using phospho-ATM-S1981 antibody.
Immunofluorescence analysis of MCF7 cell using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cell using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cell using Phospho-ATM-S1981 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
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