Accelerate Biological Research
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Our customer service representatives are available 24 hours, Monday through Friday to assist you.| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF IP ChIP |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 |
| Calculated MW | 73kDa |
| Observed MW | Refer to Figures |
| Immunogen | A synthetic peptide of human PRMT5 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | f |
| Synonym | HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5;Skb1Hs Methyltransferase |
Western blot analysis of extracts of various cell lines, using PRMT5 antibody.
Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of A549 cell using PRMT5 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cell using PRMT5 antibody. Blue: DAPI for nuclear staining.
Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.
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