Accelerate Biological Research
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Our customer service representatives are available 24 hours, Monday through Friday to assist you.| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Calculated MW | 35kDa |
| Observed MW | Refer to figures |
| Immunogen | Recombinant protein of human ETFA |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Synonym | EMA; GA2; MADD; |
Western blot analysis of extracts of various cells, using ETFA antibody.
Immunohistochemistry of paraffin-embedded human prostate using ETFA antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric using ETFA antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat brain using ETFA antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of A549 cell using ETFA antibody. Blue: DAPI for nuclear staining.
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
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