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Our customer service representatives are available 24 hours, Monday through Friday to assist you.Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 33kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human ERCC1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | e |
Synonym | UV20; COFS4; RAD10 |
Western blot analysis of extracts of various cell lines, using ERCC1 antibody.
Immunohistochemistry of paraffin-embedded rat lung using ERCC1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human rectum using ERCC1 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U2OS cell using ERCC1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using ERCC1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using ERCC1 antibody.
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
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