Accelerate Biological Research
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Our customer service representatives are available 24 hours, Monday through Friday to assist you.| Reactivity | Human |
| Tested applications | WB |
| Recommended Dilution | WB 1:500 - 1:2000 |
| Calculated MW | 37kDa |
| Observed MW | Refer to Figures |
| Immunogen | A synthetic peptide of human DFFA |
| Storage Buffer | Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | b |
| Synonym | DFF1; ICAD; DFF-45; |
Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.
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