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Tested applications | WB IHC IP ChIP |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 ChIP 1:20 - 1:100 |
Calculated MW | 83kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human CTCF |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | f |
Synonym | CTCF |
Western blot analysis of extracts of various cells, using CTCF antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using CTCF antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (40x lens).
Chromatin immunoprecipitation analysis extracts of HCT116 cell line, using CTCF rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CCCTC-binding factor (CTCF) and its paralog, the Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved transcription factors that regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs) (1-4). Although they have divergent amino and carboxy termini, both proteins contain 11 conserved zinc finger domains that work in combination to bind the same DNA elements (1). CTCF is ubiquitously expressed and contributes to transcriptional regulation of cell-growth regulated genes, including c-myc, p19/ARF, p16/INK4A, BRCA1, p53, p27, E2F1, and TERT (1). CTCF also binds to and is required for the enhancer-blocking activity of all known insulator elements and ICRs, including the H19/IgF2, Prader-Willi/Angelman syndrome, and Inactive X-Specific Transcript (XIST) anti-sense loci (5-7). CTCF DNA-binding is sensitive to DNA methylation, a mark that determines selection of the imprinted allele (maternal vs. paternal) (1). The various functions of CTCF are regulated by at least two different post-translational modifications. Poly(ADP-ribosyl)ation of CTCF is required for insulator function (8). Phosphorylation of Ser612 by protein kinase CK2 facilitates a switch of CTCF from a transcriptional repressor to an activator at the c-myc promoter (9).
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