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Our customer service representatives are available 24 hours, Monday through Friday to assist you.Reactivity | Human Mouse |
Tested applications | WB IF |
Recommended Dilution | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
Calculated MW | 53kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human CDC25C |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CDC25; PPP1R60; |
Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).
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