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Our customer service representatives are available 24 hours, Monday through Friday to assist you.Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
Calculated MW | 32kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human ATG5 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | ATG5;APG5;APG5-LIKE;APG5L;ASP;hAPG5 ; |
Immunohistochemistry of paraffin-embedded human stomach using ATG5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ATG5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ATG5 antibody at dilution of 1:100 (40x lens).
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
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