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Our customer service representatives are available 24 hours, Monday through Friday to assist you.Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 45kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human ARFGAP1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | ARF1GAP; HRIHFB2281; |
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody.
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of A549 cell using ARFGAP1 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
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